New Step by Step Map For high performance liquid chromatography
New Step by Step Map For high performance liquid chromatography
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The cellular stage carries the sample components through the column, the place they connect with the stationary section to different degrees. This conversation establishes just how long Every ingredient spends while in the column, causing their separation.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
機械的に高い圧力をかけることによって移動相溶媒を高流速でカラムに通し、これにより分析物が固定相に留まる時間を短くして分離能・検出感度を高くすることを特徴とする。
Rotating the inner valve (revealed in purple) to the inject position directs the cellular section through the sample loop and on to the column.
イオン交換クロマトグラフィーでは、無機イオンや高極性分子を電荷を利用して分離する。陽イオンタイプと陰イオンタイプの両方がある。イオン交換樹脂を利用する。
シリカゲルの粒子径が小さければ小さいほどピークの分離性は良くなるが、送液に必要なポンプの圧力が高くなる。そのため、ポンプ－インジェクター間、インジェクター－カラム間の配管の耐圧を上げたり、カラム自体を比較的高温の下にさらして溶媒の粘度を下げ、抵抗を小さくする工夫をしている。
This is a measure of the speed at which a drug is eradicated from the body. Make contact with Us Irrespective of whether you may have questions about our HPLC-MS/MS-based mostly half-existence evaluation assistance or want to discuss how we can meet your specific requirements, our staff is ready To help you. Be sure to Be happy to Make contact with us in any way you want. Our customer support Associates can be found to give you the assist you require. We sit up for hearing from you! For Research Use Only
Add a known volume of the antidepressant protriptyline, which serves as an interior standard, to each serum sample and to every external common. To eliminate matrix interferents, go a 0.5-mL aliquot of each and every serum sample or common by way of a C18 strong-phase extraction cartridge. Soon after washing the cartridge to get rid of the interferents, elute the remaining constituents, such as the analyte and The inner typical, by washing the cartridge with 0.
스포츠 도핑에서 약물 검사까지 법의독성학 응용 분야에 적용되는 방법에 대해 알아보세요.
Retention situations: Time it's going to take for each analyte to reach the detector, offering a attribute fingerprint for identification.
High-performance liquid chromatography is usually a modified and improved variety of column liquid chromatography and employs high force. HPLC is Utilized in biochemistry and analytical chemistry. This system was formulated in 1969 by Kirkland and Huber.
Prevalent mobile stage modifiers like acids and bases is usually additional to great-tune the conversation among analytes and also the column. These modifiers can:
Column variety: The stationary phase while in the column interacts with analytes. click here Using the Mistaken column chemistry may result in poor resolution. Think about using another column with a stationary phase that offers better selectivity to your analytes.
, we are able to position a solvent proportioning valve prior to one pump. The solvent proportioning price connects two or even more solvent reservoirs on the pump and determines the amount of every solvent is pulled during Just about every from the pump’s cycles. A further solution for getting rid of a pulsed circulation is to incorporate a pulse damper concerning the pump and read more also the column.